| 分类：其他 | 产品规格：50次 | 级别：试验试剂LR |
| ? | 快速——30分钟完成实验。 |
| ? | 高效分离小RNA，同时分离总RNA |
| ? | 富集200nt以下的小RNA，增加其用于下游实验的浓度。 |
| ? | 可用于miRNA、siRNA、shRNA和snRNA分析。 |
| ? | Format: Spin Column |
| ? | Sample Sources: RNA fragments (≥17 nucleotides), labeled RNA fragments, in vitrotranscription products, etc.还适用于各种来源的样品。 |
| ? | microRNA Recovery: Typically, RNA is eluted into as little as 6-10 ?l RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the supplied RB Columns is ~5 ?g. |
| ? | microRNA Purity: High quality microRNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
| ? | microRNA Storage: microRNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
| ? | Equipment Needed: Microcentrifuge for ex.eppendorf 5415c |
Descriptions
micro RNA极速提取试剂盒 provides a simple and reliable method for the rapid preparation of up to ~5 μg (per prep.) of high-quality RT-PCR-ready, DNA-free RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. The procedure is easy: treat your RNA sample with DNase I, add binding buffer, adjust the conditions by adding ethanol, and the cleaned RNA is then concentrated into ≥6 μl of RNase-free water using the RB Column. RNA fragments (≥17 bases) can be safely treated and recovered using this kit. The result is highlyconcentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc. The purification procedure after the DNase treatment typically takes about 5 minutes.
Purification of RNA samples containing genomic and plasmid DNA by using the micro RNA极速提取试剂盒. (1) RNA/DNA sample purified without prior DNase treatment, (2) DNase treated, purified RNA sample.
| Product Name | Catalog No. | Size | Price(RMB) | Protocol |
|---|---|---|---|---|
| micro RNA极速提取试剂盒 | A-R5301 | 50 次 |  | |
| A-R5302 | 200 次 |
RT-PCR相关产品全面解决方案列下：
| 产品名称 | 规格 | 操作手册 | 货号 | 询价 |
| Thermo M-MLV 反转录酶 | 10000U | A-P6801 | | |
| Thermo M-MLV 反转录酶 | 50000U | A-P6802 | ||
| Supermo III M-MLV 反转录酶 | 10000U | A-P6811 | | |
| Supermo III M-MLV 反转录酶 | 50000U | A-P6812 | ||
| Super M-MLV 反转录酶 | 10000U | A-P6502 | | |
| Super M-MLV 反转录酶 | 50000U | A-P6503 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化
